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Ires sequences12/5/2023 ![]() This permits both the gene of interest (cloned into the MCS) and the DsRed-Express2 gene to be translated from a single bicistronic mRNA.Ī copy of the pIRES2-AcGFP1 Vector product License Agreement can be found by clicking here.ĪcGFP is covered by U.S. red fluorescent protein contains the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) between the MCS and red fluorescent protein DsRed-Express2 coding region. PIRES2 DsRed-Express 2 is a eukaryotic expression vector that encodes DsRed-Express2, a variant of Discosoma sp. The DsRed-Monomer, DsRed Express, E2-Crimson and the Fruit Fluorescent Proteins are covered by one or more of the following U.S. For license information, please contact a licensing representative by e-mail at Non-Commercial Use License:Ī copy of the pIRES2 DsRed-Express2 Vector product License Agreement can be found by clicking here. For license information, please contact a licensing representative by phone at 650.919.7320 or by e-mail at Entities wishing to use this product are required to obtain a license from Takara Bio USA, Inc. Any other use of this product requires a different license from Takara Bio USA, Inc. No modifications to the product may be made without express written permission from Takara Bio USA, Inc. Such license specifically excludes the right to sell or otherwise transfer this product, its components or derivatives thereof to third parties. grants Not-For-Profit Entities a non-exclusive, non-transferable, non-sublicensable and limited license to use this product for internal, non-commercial scientific research use only. Under the Non-Commercial License, Takara Bio USA, Inc. As a condition of sale of this product to you, and prior to using this product, you must agree to the terms and conditions of the Non-Commercial License. Any and all uses of this product will be subject to the terms and conditions of the Non-Commercial Use License Agreement (the “Non-Commercial License”), a copy of which can be found below. Not-For-Profit Entities: Orders may be placed in the normal manner by contacting your local representative or Takara Bio USA, Inc. Living Colors Fluorescent Protein Products: The are several options for different fluorescent proteins, which include mCherry, ZsGreen1, and tdTomato (pLVX-IRES Vectors), and AcGFP1, DsRed2, and DsRed-Express (pIRES2 Vectors). Successfully transfected target cells are easily identified by fluorescence microscopy or flow cytometry. Similarly, fluorescent protein-containing IRES vectors allow coexpression of your target protein and a fluorescent protein. An IRES combined with a fluorescent protein Because your gene of interest and a selection marker are translated from a single RNA, you can be certain that nearly 100% of the colonies that are resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest.Īll IRES selection vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest (Rees et al. The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of the IRES from which a selection marker is expressed. pIRES vectors containing antibiotic selection markers Our pIRES vectors contain two multiple cloning sites flanking the central IRES for you to clone and express two genes of interest. Thus, two proteins are expressed simultaneously from the same bicistronic mRNA transcript. Although translation initiation of eukaryotic mRNAs occurs almost exclusively at the 5' cap, the IRES allows ribosomes to bind and initiate translation at a second, internal location. The IRES of the encephalomyocarditis virus (EMCV) permits the translation of two open reading frames from one messenger RNA. ![]() Click the "more" button for detailed information. IRES-containing bicistronic vectors allow the simultaneous expression of two proteins separately, but from the same RNA transcript (Jackson et al. ![]()
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